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Syphilis

The Syphilis TP Antibody Screen Test is lateral flow assay for the qualitative detection of antibodies to TP.

Syphilis Rapid test

Syphilis is a sexually transmitted infection caused by the bacterium Treponema-pallidum subspecies pallidum. The signs and symptoms of syphilis vary depending in which of the four stages it presents (primary, secondary, latent, and tertiary). In latent syphilis, which can last for years, there are few or no symptoms. 
Syphilis is transmitted primarily by sexual contact or during pregnancy from a mother to her fetus; the spirochete is able to pass through intact mucous membranes or compromised skin• Syphilis is a notifiable disease in many countries, including Canada the European Union, and the United States. Approximately 30% to 60% of those exposed to primary or secondary syphilis will get the disease In 2015, about 45.4 million people were infected with syphilis, with 6 million new cases• Untreated, it has a mortality of 8% to 58%, with a greater death rate in males. With early treatment, few complications result. 
Syphilis is difficult to diagnose clinically early in its presentation. Confirmation is either via blood tests or direct visual inspection using microscopy. Blood tests are more commonly used, as they are easier to perform. Because of the possibility of false positives with nontreponemal tests, confirmation is required with a treponemal test, such as treponemal pallidum particle agglutination (TPHA) or fluorescent treponemal antibody absorption test (FT A-Abs ) . Treponemal antibody tests usually become positive 2-5 weeks after the initial infection

Principle

Biologic. material Category Test duration
serum, plasma, whole blood IVD Other 15 min

The Syphilis Rapid Test Strip detects antibodies to T. pallidum (TP) through visual interpretation of color development on the internal strip. Recombinant antigens representing epitopes ofTP (15kD, l 7kD, 47kD) are immobilized on the test region of the membrane. 
During testing, the specimen reacts with recombinant TP-specific antigens conjugated to colored particles and precoated onto the sample pad of the test. The mixture then migrates through the membrane by capillary action and interacts with reagents on the membrane. If there are sufficient antibodies to TP in the specimen, a colored band will form at the test region of the membrane. 
The presence of this colored band indicates a positive result, while its absence indicates a negative result. The appearance of a colored band at the control region serves as a procedural control, indicating that the proper volume of specimen has been added and membrane wicking has occurred.