Milk Allergy Test
Allergy is a common health problem, affecting approximately 20-25% of people with immediate-type hypersensitivity reactions that manifest in the form of rhinitis, urticaria, dermatitis, gastrointestinal illness, wheezing and rarely anaphylactic shock. The term allergy is often used for type I hypersensitivity reactions (immediate typereactions), whose symptoms generally occur within 30-60 minutes after contact with the allergen. The allergens causing type I hypersensitivityreactions are mostly proteins derived from thenatural environment e.g. plant pollen, animalhair, food, mites, and insect venoms.A characteristic of type I allergies is the involvement of allergen specific immunoglobulins(antibodies) of class E (sIgE). Hence, the detection of sIgE is an important tool of modern allergy diagnostics.
A milk allergy is a food allergy, an adverse immune reaction to one or more of the constituents of milk from any animal (most commonly alpha S1-casein, a protein in cow's milk). This milkinduced allergic reaction can involve anaphylaxis, a potentially life-threatening condition .About 2.5 percent of children under three years old are allergic to milk. Nearly all infants who develop an allergy to milk do so in their first year of life. A person with milk allergy can be reactive to one of dozens of the proteins within milk. The most common one is alpha S1-casein.
|serum, plasma, whole blood
The Allergen（Milk） Rapid Test Device has been designed to detect Milk sIgE through visual interpretation of color development in the internal strip. The membrane was immobilized with streptavidin on the test region, the conjugate pad was pre-coated with colored anti-IgE antibody colloidal gold conjugates and the sample pad was pre-coated with biotinylated Milk Protein. After specimens were added, the gold-conjugates move along the membrane chromatographically by capillary action and antibodies get to the test region. If sufficient Milk sIgE is present in the sample, it will react with biotinylated Milk protein in sample pad, the mixture then migrates through conjugate pad by capillary action and interact with colored anti-IgE antibody colloidal gold conjugates, form a complex. Then the complex moves to the membrane, and combine with streptavidin. As a result, a colored band will form at the test region of the membrane. If no Milk sIgE is present in the sample, biotinylated allergen pre-coated on the sample pad will bind to streptavidin immediately, so there is no colored line at the test region of the membrane. Therefore, the colored band on the test region indicates a positive result. And appearance of a colored band at the control region serves as a procedural control. This indicates that proper volume of specimen has been added and membrane wicking has occurred.