Chlamydia Test

The genus Chlamydia includes three species: Chlamydia trachomatis, the recently described Chlamydia pneumoniae, primarily associated with humans, and Chlamydia psittasi, primarily associated with animals. Chlamydia trachomatis comprises 15 known serovars, is associated with trachomatis and gentourinary infection, and three serovars are associated with lymphogranuloma venereum (LGV). Chlamydia trachomatis infections are the most common bacterial sexually transmitted diseases. Approximately 4 million new cases occur each year in the United States, primarily cervicitis and nongonococcal urethritis. This organism also causes conjunctivitis and infant pneumonia. Chlamydia trachomatis infection has both a high prevalence and asymptomatic carriage rate, with frequent serious complications in both women and neonates. Complications of chlamydia infection in women include cervictis, urethritis, endometritis, pelvic inflammatory diseases (PID) and increased incidence of ectopic pregnancy and infertility. Vertical transmission of the disease during parturition from mother to neonate can result in inclusion conjunctivitis and pneumonia. In men, at least 40% of cases of nongonococcal urethritis are associated with chlamydia infection and epididymitis. Approximately 70% of women with endocervical infections and up to 50% of men with urethral infections are symptomatic. 

Chlamydia psittasi infection is associated with respiratory disease in individuals exposed to infected birds and is not transmitted from human to human. Chlamydia pneumonia, first isolated in 1983, is associated with respiratory infections and pneumonia. Traditionally, chlamydia infection has been diagnosed by the detection of chlamydia inclusions in tissue culture cells. Culture method is the most sensitive and specific laboratory method, but it is labour intensive, expensive, lengthy (2-3 days) and not routinely available in most institutions. Direct tests such as immunofluorescence assay (IFA) require specialized equipment and a skilled operator to read the result.

User manual

Biologic. material Category Test duration Category
swab/urine IVD Other 10 min Infectious D.

The Chlamydia Rapid Test Device (Swab/Urine) detects Chlamydia trachomatis through visual interpretation of color development on the internal strip. Antigen-specific lipopolysaccharide (LPS) monoclonal antibody is immobilized on the test region of the membrane. During testing, the specimen reacts with monoclonal anti-Chlamydia antibodies conjugated to colored particles and precoated onto the sample pad of the test. The mixture then migrates through the membrane by capillary action and interacts with reagents on the membrane. If there is sufficient chlamydia antigen in the specimen, a colored band will form at the test region of the membrane. The presence of this colored band indicates a positive result, while its absence indicates a negative result. The appearance of a colored band at the control region serves as a procedural control, indicating that the proper volume of specimen has been added and membrane wicking has occurred.